Journal: Scientific Reports
Article Title: Investigation of potential pathogenicity of Willaertia magna by investigating the transfer of bacteria pathogenicity genes into its genome
doi: 10.1038/s41598-019-54580-6
Figure Lengend Snippet: Representation of horizontal transfer analysis. Phylogenetic trees for two W. magna c2c maky proteins of putative ARB origin. The trees were constructed using maximum-likelihood method based on the leucine rich repeat sequence of W. magna . The trees were performed with 18 ( a ) and 14 ( b ) homologous sequences of W. magna retrieved by BLASTp on NCBI. In red: W. magna c2c maky leucine rich repeat-containing protein ( a ) gene 14,561; ( b ) gene 11,963); in green: the ARB ( legionella geestiana ) homologs; in blue: homologs from legionella geestiana ( a ) or homologs from giant viruses ( b ); in black: homologs from other organisms.
Article Snippet: W. magna c2c maky cells (ATCC PTA-7824) were grown at 30 °C in 175 cm2 culture flasks (Thermo Fisher Scientific, Illkirch, France) containing 75 mL of SCGYEM medium .
Techniques: Construct, Sequencing